A study on Biochemical Analysis of some traditionally used Hepatoprotective Medicinal Plants and In-Vivo assay in Rodent model

Abstract

Indigenous community/ healers have their conventional medicine system which may use a single or combination of different plant parts. The plant Morus indica used for hepatoprotective purposes by local tribes of Bodoland Territorial Area District (BTAD), Assam was well supported by the in-vivo experimental model conducted on Carbon Tetrachloride (CCl 4 ) induced acute liver damage in Wistar albino rats. Treatment with different doses of the root of Morus indica-ethanolic extracts (RoMi-EE) (100 mg and 200 mg), the elevated levels of alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) were markedly decreased and increased concentration of total cholesterol (TC), triglyceride (TG), low- density lipoprotein (LDL), very-low-density lipoprotein (VLDL), total bilirubin, gamma-glutamyltransferase (GGT), creatinine were notably checked and depleted levels of high-density lipoprotein (HDL), albumin and total protein were recovered considerably. Meanwhile, the significant increase in the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) activity and reduction of malondialdehyde (MDA) content up to 48% was noted in the RoMi-EE and silymarin treated groups. The histopathological study after treatment with different concentrations of RoMi-EE, the severity of CCl 4 induced liver and kidney intoxication was reduced in a dose-dependent manner, although the treatment with silymarin showed a much better result. In-silico molecular docking with 1NFK & 3LN1 proteins revealed that the ligand 2, 6, 10- Dodecatrien-1-ol, 3, 7, 11- trimethyl-9-(phenyl sulfonyl) (E, E)- showed best docking score (G binding affinity) of -4.95 (-45.35 kcal/mol) which is also comparable with that of silymarin and - 9.78 (-27.8172 kcal/mol) respectively. The in-vivo test so performed showed good results which are attributed to higher contents of phenolics, flavonoids, total antioxidant capacity and higher activity in total reducing power assay were obtained in the plant extract. The in-vivo test so performed showed good results which are attributed to higher contents of phenolics, flavonoids, total antioxidant capacity and higher activity in total reducing power assay were obtained in the plant extract.